Practical capillary electrophoresis
High performance capillary electrophoresis (HPCE) is the newest and perhaps most powerful separation technique available today. This single-authored text provides an integrated, comprehensive, and clearly illustrated look at the field. Users of HPCE will gain a basic understanding of principles unde...
| Main Author: | Weinberger, Robert. |
|---|---|
| Other Authors: | ScienceDirect (Online service) |
| Format: | eBook |
| Language: | English |
| Published: |
Boston :
Academic Press,
[1993]
|
| Physical Description: |
1 online resource (xv, 312 pages) : illustrations. |
| Subjects: |
| Summary: |
High performance capillary electrophoresis (HPCE) is the newest and perhaps most powerful separation technique available today. This single-authored text provides an integrated, comprehensive, and clearly illustrated look at the field. Users of HPCE will gain a basic understanding of principles underlying electrophoresis and go on to learn about mode selection, methods development, detection, and quantative analysis. Ideally suited for analytical chemists and analytical biochemists with applications involving small molecules, proteins, peptides, DNA, and ion separations, this book provides a comparative assessment of related techniques. The author is an internationally recognised scientist and serves as the instructor for short courses on HPCE as offered by the American Chemical Society. * Stresses basic principles and applications * Helps users select appropriate HPCE modes and develop methods * Describes how to perform quantitative analyses * reinforces concepts with clear illustrations. |
|---|---|
| Item Description: |
Includes bibliographical references and index. Introduction -- Electrophoresis -- Microchromatographic separation methods -- Capillary electrophoresis -- Historical perspective -- Instrumentation -- Sources of information on HPCE -- Capillary electrophoresis: a family ot techniques -- Basic concepts -- Electrical conduction in fluid solution -- Language of electrophoresis -- Electroendoosmosis -- Efficiency -- Resolution -- Joule heating -- Optimizing the voltage and temperature -- Capillary diameter and buffer ionic strength -- Optimizing the capillary length -- Buffers -- Capillaries -- Capillary zone electrophoresis -- Mobility -- Buffer additives -- Solute-wall interactions -- Bandbroadening -- Applications -- Methods development -- Capillary isoelectric focusing -- Capillary coatings and electroosmotic flow -- Performing a run -- Focusing and mobilization problems -- Applications -- Size separations in capillary gels and polymer networks -- Separation mechanism -- Materials for CGE -- Applications -- Pulsed-field capillary electrophoresis -- Future of CGE -- Capillary isotachophoresis -- Separation mechanism -- Instrumental aspects -- Buffer selection. (cont) Advantages and problems with CITP -- Applications -- Electrokinetic capillary chromatography -- Micelles -- Separation mechanism -- Sources of bandbroadening -- Selecting the electrolyte system -- Elution range of MECC -- Alternative surfactant systems -- Applications and methods development -- Cyclodextrins -- Chiral recognition -- Optimization -- Capillary electrochromatography -- Electroosmotic pump -- Bandbroadening -- Applications -- Electrochromatography in perspective -- Injection -- Volumetric constraints on injection size -- Performing an injection and run -- Injection techniques -- Injection problems and solutions -- Alternative injection schemes -- Stacking -- Other enrichment techniques -- Detection -- On-capillary detection -- Detection problem -- Classes of detectors -- Limits of detection -- Bandbroadening -- Absorption detection -- Fluorescence detection -- Indirect detection -- Mass spectrometry -- Other detection techniques -- Fraction collection -- Putting it all together -- Selecting the mode of HPCE -- Requirements for robust separations. (cont) Realistic compromises -- Linear dynamic range -- Sample preparation -- Quantitative analysis -- Correction factors -- Special topics -- Field effect electroendoosmosis -- Ultrafast separations -- HPCE on a chip -- Multiple capillary systems -- Gradient and programmed systems -- Two-dimensional systems -- Immunologic trace enrichment. Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL. High performance capillary electrophoresis (HPCE) is the newest and perhaps most powerful separation technique available today. This single-authored text provides an integrated, comprehensive, and clearly illustrated look at the field. Users of HPCE will gain a basic understanding of principles underlying electrophoresis and go on to learn about mode selection, methods development, detection, and quantative analysis. Ideally suited for analytical chemists and analytical biochemists with applications involving small molecules, proteins, peptides, DNA, and ion separations, this book provides a comparative assessment of related techniques. The author is an internationally recognised scientist and serves as the instructor for short courses on HPCE as offered by the American Chemical Society. * Stresses basic principles and applications * Helps users select appropriate HPCE modes and develop methods * Describes how to perform quantitative analyses * reinforces concepts with clear illustrations. Use copy Restrictions unspecified. star MiAaHDL. Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010. MiAaHDL. |
| Physical Description: |
1 online resource (xv, 312 pages) : illustrations. |
| Format: |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |
| Bibliography: |
Includes bibliographical references and index. |
| ISBN: |
9780323140393 0323140394 |