Electrophoresis a survey of techniques and applications. part A, Techniques /
| Other Authors: | Deyl, Zdeněk., ScienceDirect (Online service) |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
Amsterdam ; New York :
Elsevier Scientific Pub. Co.,
1979-1983.
|
| Physical Description: |
1 online resource (xiv, 390 pages) : illustrations. |
| Series: |
Journal of chromatography library ;
v. 18. |
| Subjects: |
Table of Contents:
- Front Cover; Electrophoresis: A Survey of Techniques and Applications; Copyright Page; Contents; Contributors; Preface; Introduction; Chapter 1. Theory of electromigration processes; Introduction; Equilibria in electrophoretic systems; Processes in electrophoretic systems; Mathematical description of the electrophoretic process; References; Chapter 2. Classification of electromigration methods; Introduction; Zone electrophoresis; The moving boundary method; Isotachophoresis; Focusing methods; Combined methods; References; Chapter 3. Evaluation of the results of electrophoretic separations.
- IntroductionDetermination of mobility; Principles of quantitative evaluation; Chapter 4. Molecular size and shape in electrophoresis; Introduction; Elimination of charge differences; Choice of standard series; Molecular size determination by pore limit electrophoresis; Determination of Stokes radii by rheophoresis; References; Chapter 5. Zone electrophoresis (except gel-type techniques and immunoelectrophoresis); Paper techniques; Thin-layer electrophoresis; References; Chapter 6. Gel-type techniques; Introduction; Theory of gel electrophoresis; Starch gel electrophoresis.
- Acrylamide gel electrophoresisAgarose gel electrophoresis; Agarose-acrylamide composite gels; References; Chapter 7. Quantitative immunoelectrophoresis; Introduction; Chemicals and solutions; Apparatus and accessories; Practical application; References; Chapter 8. Moving boundary electrophoresis in narrow-bore tubes; Introduction; Principles of moving boundary electrophoresis; Practical application; State of the art and comparison with other techniques; References; Chapter 9. Isoelectric focusing; Principle; Theoretical aspects; Carrier ampholytes; Support media; Gel tubes; Thin layers.
- Density gradientFree solution; Two-dimensional methods; lmmunoisoelectric focusing; Transient state isoelectric focusing; Conclusions; References; Chapter 10. Analytical isotachophoresis; Introduction; Theoretical; Quality and quantity in isotachophoresis; Instrumentation; Practical application; References; Chapter 11. Continuous flow-through electrophoresis; Introduction; General aspects; Electrophoretic cells of other shapes; Factors affecting zone width in flow-through cells; References; Chapter 12. Continuous flow deviation electrophoresis; Introduction.
- Theoretical aspects of continuous flow deviation electrophoresisModes of implementation of continuous flow deviation electrophoresis; Flat fluid band electrophoresis (""free-flow"" electrophoresis); Endless fluid belt electrophoresis; Conclusion; Acknowledgements; Symbols and units; References; Chapter 13. Preparative electrophoresis in gel media; Introduction; Gel composition and concentration; Gel dimensions and sample load; Voltage gradient and the electrophoresis time; Duration of electrophoresis and flow-rate; References; Chapter 14. Preparative electrophoresis in columns; Introduction.