Search Results - "X-ray crystallography"

Crystallography made crystal clear : a guide for users of macromolecular models 3rd ed. by Rhodes, Gale

Subjects: “…X-ray crystallography.…”
Elsevier - Click here for access

Biomolecular crystallography : principles, practice, and application to structural biology by Rupp, Bernhard

Subjects: “…X-ray crystallography.…”

Structure-based drug discovery by SpringerLink (Online service)

Contents: “…Front Matter; Five Years of Increasing Structural Biology Throughput -- A Retrospective Analysis; Protein Production for Structural Genomics -- Strategies for the Next Phase; Introduction to Fragment Screening; Fragment-Based NMR Screening in Lead Discovery; Fragment-Based Screening by X-ray Crystallography; Scaffold-Based Drug Discovery; Biophysical Methods; Illustration of Current Challenges in Molecular Docking; Scoring Functions; Back Matter.…”
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Structural genomics. Part A 1st ed. by ScienceDirect (Online service)

Contents: “…Introduction; Analysis of genomes for structural genomics and the limitations of sequence analysis; Structure determination pipeline for structural genomics;Gene cloning and protein expression;Gene cloning and expression of membrane proteins; High-throughput protein purification for x-ray crystallography and NMR; High-throughput protein crystallization; Structure determination using synchrotron radiation; Application of NMR to structural genomics; Development of key high-throughput technologies: High-throughput functional screening; Development of key high-throughput technologies: Structure prediction and homology modeling; Functional inferences from structure; Structural genomics programs (SARS case); Structural genomics programs (E.coli);Structural genomics as a structural foundation for drug discovery; Structural genomics of eukaryote; Structural genomics of complexes; Dissemination structural genomics data to biology community; ; ; ;…”
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Advances in protein molecular and structural biology methods by ScienceDirect (Online service)

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Crystallography of protein dynamics by ScienceDirect (Online service)

Contents: “…8 Software availability -- Acknowledgements -- References -- Chapter Eight: Refinement of multiconformer ensemble models from multi-temperature X-ray diffraction dataRefinement of multiconformer ensemble models -- 1 Introduction -- 2 Collection of multi-temperature X-ray diffraction data -- 2.1 Obtaining crystals for X-ray diffraction -- 2.2 Achieving high-temperature capabilities and temperature control -- 2.3 Diffraction data collection -- 2.4 Data processing -- 3 Single conformer model refinement -- 3.1 Molecular replacement -- 3.2 Initial model building -- 3.3 Iterative model refinement -- 3.4 Modeling an unknown ligand appearing at high temperatures -- 4 Multiconformer model refinement -- 4.1 Automatic refinement using qFit -- 4.2 Manual pruning and refinement -- 4.3 Automatic relabeling of structural segments -- 4.4 Modeling coupled conformational preferences -- 5 Identifying temperature-dependent conformational changes -- 5.1 Ringer analysis -- 6 Summary and conclusions -- Acknowledgments -- References -- Chapter Nine: Combining temperature perturbations with X-ray crystallography to study dynamic macromolecules: A thorough discussion of experimental methods -- 1 Introduction -- 2 Sample cryocooling in macromolecular crystallography -- 2.1 Cryocooling has become standard practice in macromolecular crystallography -- 2.2 Cryocooling can cause lattice distortions that lead to practical challenges -- 2.3 Cryocooling perturbs the structures of macromolecules -- 3 Non-cryogenic macromolecular crystallography -- 3.1 Modern hardware and software obviate the need for cryocooling -- 3.1.1 Breakthroughs in X-ray detector technology -- 3.1.2 Integration of weak reflection intensities with modern software -- 3.2 Practical considerations for non-cryogenic data collection -- 3.2.1 Sample preparation -- 3.2.2 Data collection.…”
Elsevier - Click here for access