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Single-molecule metal-induced energy transfer from basics to applications /

This thesis presents a novel single-molecule spectroscopy method that, for the first time, allows the dipole orientations and fluorescence lifetimes of individual molecules to be measured simultaneously. These two parameters are needed to determine the position of individual molecules with nanometer...

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Main Author: Karedla, Narain,
Other Authors: SpringerLink (Online service)
Format: eBook
Language: English
Published: Cham, Switzerland : Springer, 2017.
Physical Description: 1 online resource.
Series: Springer theses.
Subjects:
Summary: This thesis presents a novel single-molecule spectroscopy method that, for the first time, allows the dipole orientations and fluorescence lifetimes of individual molecules to be measured simultaneously. These two parameters are needed to determine the position of individual molecules with nanometer accuracy near a metallic structure. Proof-of-principle experiments demonstrating the value of this new single-molecule localization concept are also presented. Lastly, the book highlights potential applications of the method in biophysics, molecular physics, soft matter and structural biology.
Item Description: "Doctoral thesis accepted by the Göttingen University, Göttingen, Germany."
Supervisor's Foreword; Abstract; Acknowledgements; Contents; 1 Introduction; References; 2 Theory; 2.1 Quantum Mechanical Picture of Fluorescence; 2.1.1 Molecular Excitation and Emission; 2.1.2 Single-Singlet and Singlet-Triplet Transitions; 2.1.3 Franck-Condon Principle; 2.1.4 Radiationless De-Excitation; 2.1.5 Einstein's Coefficients and Spontaneous Emission Rate; 2.2 Plane Waves and Maxwell's Equations; 2.3 Fresnel's Equations; 2.3.1 Total Internal Reflection; 2.3.2 Thin Layers and Frustrated Internal Reflection; 2.3.3 Fresnel's Equations for a Metal Surface; 2.4 The Oscillating Dipole.
2.4.1 Dipole in a Homogeneous Environment2.4.2 Dipole on a Planar Dielectric Interface; 2.4.3 Dipole on a Metal Surface; References; 3 Single-Molecule Metal-Induced Energy Transfer (smMIET); 3.1 Experimental Setups; 3.1.1 MicroTime 200 Setup; 3.1.2 Wide-Field Setup for Defocused Imaging; 3.2 Proof of Principle Experiments; 3.2.1 Substrate Preparation; 3.2.2 Results; 3.2.3 Discussion and Outlook; References; 4 Single-Molecule Transition Dipole Imaging; 4.1 Radially Polarized Laser Excitation; 4.1.1 Excitation Patterns; 4.1.2 Experimental Setup; 4.1.3 Single-Molecule Excitation Images.
4.1.4 Pattern Matching4.1.5 Multidimensional Emitters; 4.2 smMIET with Radially Polarized Excitation; 4.2.1 Methods; 4.2.2 Results and Discussion; 4.3 Defocused Imaging; 4.3.1 Theory; 4.3.2 Experimental Setup; 4.3.3 Pattern Matching and Lateral Localization; 4.3.4 Multidimensional Emitters; 4.4 Excitation-Emission Transition Dipole Imaging; 4.4.1 Experimental Setup and Methods; 4.4.2 Results; 4.4.3 Discussion and Outlook; 4.5 Transition Dipole Imaging of Carbon Nanodots; References; 5 Discussion and Outlook; 5.1 MIET on Metal Thin Films; 5.2 SmMIET for Structural Biology.
5.3 Dynamics Using MIET -- DynaMIET5.4 Ongoing Experiments; References; 6 Conclusion; References; Glossary; Appendix Curriculum Vitae.
Includes bibliographical references.
This thesis presents a novel single-molecule spectroscopy method that, for the first time, allows the dipole orientations and fluorescence lifetimes of individual molecules to be measured simultaneously. These two parameters are needed to determine the position of individual molecules with nanometer accuracy near a metallic structure. Proof-of-principle experiments demonstrating the value of this new single-molecule localization concept are also presented. Lastly, the book highlights potential applications of the method in biophysics, molecular physics, soft matter and structural biology.
Physical Description: 1 online resource.
Bibliography: Includes bibliographical references.
ISBN: 9783319605371
3319605372